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In 2014, the Office of Naval Research embarked on a four-year, $3.85 million research program to explore the phenomena it calls premonition and intuition, or “ MULTISTRAP LOGO POOL SLIDE Mules black In China Sale Online Clearance Store Cheap Price Authentic Sale Online New Arrival Fashion RwIc3Bf
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“We have to understand what gives rise to this so-called ‘sixth sense,’ says Peter Squire, a program officer in ONR’s Expeditionary Maneuver Warfare and Combating Terrorism department. Today’s Navy scientists place less emphasis on trying to understand the phenomena theoretically and more on using technology to examine the mysterious process, which Navy scientists assure the public is not based on superstition. “If the researchers understand the process, there may be ways to accelerate it — and possibly spread the powers of intuition throughout military units,” says Dr. Squire. The Pentagon ’s focus is to maximize the power of the sixth sense for operational use. “If we can characterize this intuitive decision-making process and model it, then the hope is to accelerate the acquisition of these skills,” says Lieutenant Commander Brent Olde of ONR’s Warfighter Performance Department for Human and Bioengineered Systems. “[Are] there ways to improve premonition through training?” he asks.

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According to the Pentagon, the program was born of field reports from the war theater, including a 2006 incident in Iraq, when Staff Sergeant Martin Richburg, using intuition, prevented carnage in an IED, or improvised explosive device, incident. Commander Joseph Cohn, a program manager at the naval office, told the New York Times, “These reports from the field often detailed a ‘sixth sense’ or ‘Spidey sense’ that alerted them to an impending attack or I.E.D., or that allowed them to respond to a novel situation without consciously analyzing the situation.”

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Subdivision: Oak Tree
County: Montgomery

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Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model

Soriano, Perry A.; Liu, Nian; Castillo, Erick; Foster, Brock; Artinyan, Avo; Kim, Joseph; Huang, Wendong; Wagman, Lawrence D.

Introduction. We examined the murine hepatectomy model of liver regeneration (LR) in the setting of neoadjuvant chemotherapy. Methods. C57BL/6 mice were randomized to receive neoadjuvant intraperitoneal (IP) injections of a control, oxaliplatin (15 mg/kg), or irinotecan (100 mg/Kg or 250 mg/Kg) solution. Hepatectomy (70%) was performed 14 days after the final IP treatment. Animals were sacrificed at postoperative day (D) 0, 1, 2, 3, and 7. Liver remnants and serum were collected for analysis. T-tests for independent samples were used for statistical comparisons. Results. For oxaliplatin, percent LR did not differ at D1 or D2 but was significantly less at D3 (89.0% versus 70.0%, P = 0.048) with no difference on D7 (P = 0.21). Irinotecan-treated mice at both dose levels (100 mg/Kg and 250 mg/Kg) showed no significant differences in LR. BrdU incorporation was significantly decreased in oxaliplatin-treated animals (D1,2,3). Conclusions. Neoadjuvant oxaliplatin but not irinotecan impairs early LR in a posthepatectomy murine model which correlates with decreased DNA synthesis. PMID:22164336

Monoclonal antibodies against the rat liver glucocorticoid receptor.

Okret, S; Wikström, A C; Wrange, O; Andersson, B; Gustafsson, J A


Splenic cells from one BALB/c mouse and one C57/BL mouse, immunized with purified rat liver glucocorticoid receptor (GR), were fused with the mouse myeloma cell line Sp 2/0-Ag 14. Screening for production of anti-GR-antibodies by the hybridomas was carried out with an enzyme-linked immunosorbent assay, using partially purified rat liver GR as antigen. Further screening was by a second-antibody immunoprecipitation assay using [3H]triamcinolone acetonide-GR complex from rat liver cytosol as tracer. Hybridomas from 10 different microplate wells, positive in both assays, were successfully cloned by the limiting dilution method to monoclonality. The different origins of the monoclonal antibodies were confirmed by their various isoelectric points when analyzed by isoelectric focusing. Four of the monoclonal hybridoma cell lines secreted IgM antibodies; two, IgG1; three, IgG2a; and one, IgG2b. The GR-antibody complex was identified in glycerol density gradients by a shift of the 4S GR to an 8.5S or 19S GR-antibody complex when incubated with monoclonal IgG or IgM antibody, respectively. The 10 monoclonal antibodies recognized different determinants on the GR, all situated on that domain of the receptor that is separate from the ligand and DNA-binding domains. Also, the cross-reactivity to the mouse liver GR varied among the monoclonal antibodies. No cross-reactivity was observed to the human lymphocytic GR. NaDodSO4 electrophoresis of a 0.5% pure GR preparation followed by immunoblotting using one of the monoclonal antibodies identified a single peptide with a molecular weight of 94,000, identical to the purified rat liver GR. Images PMID:6200880

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Jonassen, T E; Christensen, S; Kwon, T H; Langhoff, S; Salling, N; Nielsen, S


The present study was performed to investigate the renal handling of water in rats with decompensated liver cirrhosis. Liver cirrhosis was induced by intraperitoneal administration of carbon tetrachloride twice weekly for 16 wk. Control rats were treated with vehicle. The cirrhotic rats developed severe disturbances in water homeostasis: urine production was decreased and hyperosmotic, the rats had significantly decreased plasma sodium concentration and ascites, and the ability to excrete an intravenous water load was significantly impaired. Plasma concentrations of vasopressin and aldosterone were increased. Mean arterial pressure, glomerular filtration rate (GFR), and fractional lithium excretion were decreased. Acute vasopressin type 2-receptor blockade with the selective nonpeptide antagonist OPC-31260 (800 microg. kg(-1). h(-1)) was performed during conditions whereby volume depletion was prevented by computer-driven, servo-controlled intravenous volume replacement with 150 mM glucose. The aquaretic response to OPC-31260 was similar in cirrhotic and control rats . However, the OPC 31260-induced rises in fractional water excretion (delta V/GFR; +24%) and fractional distal water excretion (delta V/C(Li); +46%) were significantly increased in the cirrhotic rats , where V is flow rate and delta is change. This suggests that vasopressin-mediated renal water reabsorption capacity was increased in the cirrhotic rats . Semiquantitative immunoblotting revealed that the expression of the vasopressin-regulated water channel aquaporin-2 was unchanged in membrane fractions of both whole kidney and inner medulla from cirrhotic rats . Together, these results suggest a relative escape from vasopressin on collecting duct water reabsorption in rats with decompensated liver cirrhosis.

In vitro callus and in vivo leaf extract of Gymnema sylvestre stimulate β-cells regeneration and anti-diabetic activity in Wistar rats .

Ahmed, A Bakrudeen Ali; Rao, A S; Rao, M V


A methanol extract of Gymnema sylvestre leaf and callus showed anti-diabetic activities through regenerating β-cells. Optimum callus was developed under stress conditions of blue light with 2,4-D (1.5 mg/l) and KN (0.5 mg/l), which induced maximum biomass of green compact callus at 45 days, as determined by growth curve analysis. Leaf and optimum callus extracts contains gymnemic acid, which was analyzed using TLC, HPTLC and HPLC methods. The research reported here deals with leaf and callus extracts of G. sylvestre, which significantly increase the weight of the whole body, liver , pancreas and liver glycogen content in alloxan-induced diabetic rats (Wistar rats ). The gymnemic acid of leaf and callus extracts significantly increases the regeneration of β-cells in treated rats , when compared with the standard diabetic rats . It could have potential as a pharmaceutical drug for insulin-dependent diabetes mellitus (IDDM). Copyright © 2010 Elsevier GmbH. All rights reserved.

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